Part:BBa_K565004
Microcin C51 production device
Microcin C51 is a bacteriocin protein produced by the Escherichia coli pMccC51 plasmid. They make holes in bacterial membranes.
In our project, we used Microcin C51 to kill pathogen cells (in our case they were E. coli DH5alpha strain) by membrane lysis, disinfecting the contaminated water.
Bibliography
Veselovskii. A. M. et al. Activation of the Expression of the Microcin C51 Operon upon Glucose Starvation of Cells at the Exponential Growth Phase. Russian Journal of Genetics, Vol. 41, No. 1, 2005, pp. 40–43. Translated from Genetika, Vol. 41, No. 1, 2005, pp. 48–52.
Fomenko. D. et al. Regulation of microcin C51 operon expression: the role of global regulators of transcription. Res. Microbiol. 152 (2001) 469–479.
Severinov K. et al. Class I Microcins: Their Structures, Activities, and Mechanisms of Resistance. 2011, Part 5, 289-308.
Metlitskaya A.Z. et al. Structure of microcin C51, a new antibiotic with a broad spectrum of activity. FEBS Letters 357 (1995) 235-238.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 4082
Illegal PstI site found at 759
Illegal PstI site found at 1239
Illegal PstI site found at 3088
Illegal PstI site found at 4334 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 4082
Illegal PstI site found at 759
Illegal PstI site found at 1239
Illegal PstI site found at 3088
Illegal PstI site found at 4334 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 4082
Illegal BglII site found at 1399 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 4082
Illegal PstI site found at 759
Illegal PstI site found at 1239
Illegal PstI site found at 3088
Illegal PstI site found at 4334 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 4082
Illegal PstI site found at 759
Illegal PstI site found at 1239
Illegal PstI site found at 3088
Illegal PstI site found at 4334 - 1000COMPATIBLE WITH RFC[1000]
| n/a | Microcin C51 production device |